Product Name: Akt (Ser-473), phospho-specific
Type: Mouse Monoclonal IgG1
Elisa: 1:2000
WB: 1:250
Background: Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen syn
CAS NO.: 1439399-58-2
CB-839
Immunogen: Clone M114 was generated from a peptide containing amino acid residues surrounding Serine 473 in human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.
Buffer And Storage: Mouse monoclonal purified with protein G chromatography is supplied in 200μl phosphate-buffered saline with 0.02% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.
Specificity: This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Akt on SDS-PAGE immunoblots of mouse NIH3T3 cells treated with PDGF and human A431 cells treated with EGF.
PubMed ID:http://aac.asm.org/content/50/9/2932.abstract