Product Name: Cdk1 (Tyr-15)[conserved site], phospho-specific
Type: Mouse Monoclonal IgG1
Elisa: 1:1000
WB: 1:250
Background: Cyclin-dependent kinases (Cdks) are a family of serine/threonine kinases that require association with regulatory subunits known as cyclins for activation. In addition, post-translational phosphorylation and dephosphorylation events regulate Cdk activity.
CAS NO.: 1194044-20-6
LY2811376
Immunogen: Clone M231 was generated from a phospho-Cdk1 (Tyr-15) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Tyr-15 in human Cdk1. This sequence is conserved in rat and mouse Cdk1, and is highly conserved in other Cdks, in
Buffer And Storage: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity: The antibody detects a 34 kDa* band corresponding to Cdk1 on SDS-PAGE immunoblots of human SYF and HeLa cells, and this band is removed after alkaline phosphatase treatment. The antibody may also detect Tyr-15 phosphorylation in Cdk2, Cdk3, Cdk5, and Cdk6
PubMed ID:http://aac.asm.org/content/50/11/3695.abstract