Way were identified by KEGG enrichment annotation (Fig. 4: 5). KEGG evaluation showed
Way have been identified by KEGG enrichment annotation (Fig. 4: 5). KEGG analysis showed that compared with CAK (BR spraying for 0 h), the expression on the UTPglucose-1-phosphate uridylyltransferase (UGP), SPS, glucose-6-phosphate isomerase (GPI), pyrophosphateJin et al. BMC Genomics(2022) 23:Web page 10 ofFig. 5 A doable model of the BR signaling pathway with BRs (the activation state of BR signaling) sprayed onto tea leavesJin et al. BMC Genomics(2022) 23:Page 11 offructose-6-phosphate 1-phosphotransferase (PFP), and epidermis-specific secreted glycoprotein (EP) important regulatory genes related for the sucrose biosynthesis pathway were upregulated after BR spraying for three h, 9 h, 24 h, and 48 h.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes in the biosynthetic pathway of flavonoidsEleven genes involved in flavonoid biosynthesis had been identified by KEGG enrichment annotation (Fig. four: six). The flavonoid biosynthesis-related genes PAL, C4H, 4CL, chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3,5-hydroxylase (F3’5’H), DFR, LAR, ANR, and UFGT had been upregulated, with peak values observed at 48 h.DiscussionBR signal transduction mechanism in tea leavesThrough KEGG enrichment and annotation, 26 genes involved within the BR signal transduction pathway have been identified. As outlined by the heat maps of genes related to BR signal transduction beneath distinct BR treatment options, it was identified that 26 genes in the BR signal transduction pathway have been drastically upregulated with Duocarmycins drug growing BR spraying time. Combined KDM5 manufacturer together with the BR signal transduction maps of Arabidopsis and rice, we describe a probable model from the BR signal pathway in tea leaves [291] (Fig. 5). At present, the signal transduction pathway of BR in Arabidopsis and rice has been reported. Compared with rice, the signal transduction pathway of BR in tea leaves is related to that of Arabidopsis [24]. Unlike the BR signal transduction pathway inside a. thaliana, BAK1-like kinase consists of both SERK and TMK4 in the BR signal transduction pathway of tea leaves. In our transcriptome data, the ATBS1-interacting factors (AIF) and paclobutrazol resistance 1 (PRE) genes did not significantly differ in expression levels, whereas that in the teosinte branched (TCP) gene was important. AIF will be the adverse regulator of BR signal transduction, while PRE and TCP will be the good regulators of BR signal transduction [34]. The outcomes showed that TCP, the forward regulator of BR signal transduction, plays a top role in the effects in the exogenous spraying of BRs onto young tea leaves.Exogenous spraying of BR promotes the growth and improvement of tea plantsGBSS, and SBE genes connected to starch synthesis; and the flavonoid biosynthesis-related PAL, C4H, 4CL, CHS, CHI, F3H, F3’5, DFR, LAR, ANR, and UFGT genes were identified. The results showed that exogenous spraying of BRs upregulated the expression of genes associated to sucrose synthesis, chlorophyll synthesis, starch synthesis, and flavonoid biosynthesis. It might be inferred that exogenous BR spraying enhanced the content of sucrose, chlorophyll, starch, and flavonoids. Furthermore, a large number of very expressed cyclin genes, which includes Cyc, CycD3, CycD4, and CDC6, had been found. Cell cycle regulatory proteins can bind to cell differentiation cycle-coding proteins and activate corresponding protein kinases, thus promoting cell division. BRs can enhance plant growth by advertising cell division.