The ongoing threat of infectious disease outbreaks demands diagnostic tools that are not only fast and accurate but also sustainable, cost-effective, and deployable in diverse environments. This study presents a scalable, eco-friendly paper-based platform—HiPAD—engineered for rapid, visual detection of viral pathogens using loop-mediated isothermal amplification (LAMP). The system integrates a rGO/MWCNTs nano-circuit heater fabricated via a simple, reproducible suction filtration process, enabling efficient on-chip heating without reliance on bulky or expensive equipment.
The electrothermal performance of the hybrid material is critically dependent on the optimal balance between rGO and MWCNTs. At 5 wt% rGO loading, the composite achieves maximum electrical conductivity, forming a robust percolating network that facilitates uniform electron transfer and rapid Joule heating. When connected to a 5 V lithium battery, the heater reaches stable temperatures above 70 °C within 10 seconds, sufficient to initiate and sustain LAMP reactions at 60–65 °C. Infrared thermal imaging confirms consistent heat distribution across the reaction chamber, while finite element simulations validate the thermal homogeneity of the liquid phase, ensuring reliable amplification conditions.
The HiPAD was validated using SARS-CoV-2 N gene templates across a broad dynamic range from 2.5 × 10¹⁰ to 2.5 copies mL⁻¹. After 45 minutes of incubation, positive samples displayed a distinct dusty-blue color change due to hydroxynaphthol blue (HNB) dye interaction with amplified DNA, while negative controls remained purple. The detection limit of 25 copies mL⁻¹ was achieved—40 times more sensitive than conventional real-time PCR instruments. Gel electrophoresis confirmed the presence of characteristic ladder-like amplicons in all positive samples, validating both visual and molecular outcomes.Thiomorpholine 1,1-dioxide custom synthesis
A defining feature of this platform is its environmental sustainability.M-CSF Protein, MouseStorage & Stability The entire paper-based chip is designed for single-use disposal and can be safely incinerated after testing, eliminating biohazard risks and preventing secondary transmission—especially crucial during pandemics.PMID:34862760 Reusable components, including the heater, electrode plate, and sealing glass, reduce long-term waste and operational costs. The device operates under ambient conditions with no need for refrigeration or specialized infrastructure.
Furthermore, the modular architecture allows straightforward expansion into multiplex formats (M-HiPAD), enabling simultaneous detection of multiple pathogens using distinct primer sets in separate chambers. This enhances its utility in outbreak monitoring, antimicrobial resistance surveillance, and comprehensive public health screening.
In conclusion, the HiPAD platform exemplifies a new generation of diagnostic devices: high-performing, low-cost, and environmentally responsible. By leveraging advanced nanomaterials in a simple, scalable format, it delivers laboratory-quality sensitivity and speed in a field-ready, disposable package. Its potential for widespread deployment in resource-limited settings makes it a transformative solution for global health equity, pandemic preparedness, and decentralized diagnostics.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com