Product Name: ROCK-I (C-terminal), cleavage-specific
Type: Mouse Monoclonal IgG1
Elisa: 1:500
WB: 1:250
Background: Rho-associated kinases (ROCKs) are a family of serine/threonine kinases that have important roles in Rho-mediated signaling. The structure of ROCKs (ROCK-I/ROKβ and ROCK-II/ROKα) includes an N-terminal catalytic domain, a coiled-coil region, an
CAS NO.: 1093403-33-8
SRT 2104
Immunogen: Clone (154C1465) was generated from a.a. 1114-1126 adjacent to the caspase cleavage site in human ROCK-I. This sequence has high homology to rat and mouse ROCK-I, and has significant homology to the conserved region in ROCK-II.
Buffer And Storage: Mouse monoclonal purified with protein G chromatography is supplied in 200μl phosphate-buffered saline with 0.2% gelatin and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.
Specificity: The antibody detects a 31 kDa* band corresponding to the C-terminal cleavage fragment of ROCK-I on SDS-PAGE immunoblots of human Jurkat cells treated with camptothecin.
PubMed ID:http://aac.asm.org/content/51/3/1112.abstract