Ret any inconvenience that our customers may incur as a result. Please check our web site for up-to-date information about supplies and for the name and contact information of the responsible person at Isis.
The Glen Report 13.1
In this issue, we fortunately have lots of good news to go with the preceding announcement. We have another fine article from Misha Shchepinov from his work in Oxford. This time, he discusses a resurrection of the trityl group with applications in fluorescence, combinatorial chemistry and mass spectroscopy. We would appreciate your input as to which of the products discussed should be made commercially available. In addition, beginning on Page 2, Scott Strobel has kindly updated us on the state of play with Nucleotide Analog Interference Mapping (NAIM) and the newer, more advanced Interference Suppression. And it would not be The Glen Report if there were no new products to discuss, so here we go.
3′-Amino-Modifier C7 CPG 1000
Over the last 18 months, we have been changing our support for minor bases and modifiers to 1000CPG. This change has been made to allow longer oligos to be routinely prepared. The analytical information has NAIM Update been amended to include a test to determine where Novel Trityl Derivatives the drop-off point for each PolyPlex Reagents specific lot occurs. We think this is more New Monomers relevant than the pore size of the
(Continued on Page 13)
VOLUME 13 NUMBER 1 AUGUST 2000
New Amino-Modifiers
A Rapid Method for Atomic Mutagenesis of Nucleic Acids
Interference Mapping
Nucleotide Analog Interference Mapping (NAIM) is a powerful new approach to perform atomic mutagenesis simultaneously, yet individually, at every position in an RNA using materials easily prepared by in vitro transcription.1 Through the use of a collection of nucleotide analogs, now available from Glen Research, it is possible to assess the importance of every 2′-OH, exocyclic amine, or imino group throughout the length of an RNA. By comparing the results of multiple nucleotide analogs, it is possible to determine which ribose rings adopt unusual sugar puckers, which 2′-OH groups serve as hydrogen bond donors and/or acceptors, which nucleotides have perturbed pKas for potential use in folding or catalysis. Furthermore, this approach can reveal which nucleotides make tertiary contacts, on which nucleotide face the contacts are made, and where metal ions are coordinated within an RNA fold.51298-62-5 manufacturer This extraordinary level of chemical detail is available quite rapidly using a collection of nucleotide analogs that modify individual functional groups on the RNA base or ribose sugar. The analogs are all -thiotriphosphates which mark the sites of their incorporation within the transcript with an phosphorothioate linkage2.54197-31-8 supplier This linkage allows simple iodine cleavage and makes it possible to identify the sites where incorporation of the analog interfered with or enhanced the activity of the RNA molecule.PMID:20301656 The magnitude of an interference effect at each position in the molecule can be quantified by simply viewing a sequencing gel of the reaction products (Figure 1A). A complete description of this approach was the subject of Glen Report, 1998, 11(2), 6-8, and can be accessed at:
http://glenres/ProductFiles/ Information/Strobel.HTML
An up-to-date listing of the available -thiotriphosphates is maintained at:
http://glenres/catalog/NAIM.html
This rapid and inexpensive alternative to single site atomic mutagenesis has been implemented for the study of seve.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com