E measured 5 times and the result showed the alterations in opacity measurements at the intermediate and high frequency ranges which represents the difference among the membrane composition and cytosolic conductance on the exosomes. Summary/Conclusion: A new class of electrical impedance measurement technique was developed with the capability to characterize and distinguish exosomes primarily based on their exclusive CD49d/Integrin alpha 4 Proteins manufacturer dielectric properties as their biogenesis was subjected to systematic modifications beneath diverse culture circumstances. This approach is usually additional utilized for classification of exosomes primarily based on their cell of origin and can be evolved as a diagnostic tool for characterizing the pathogenic exosomes. Funding: UC Faculty Development Fund.OF22.Characterization of exosomes-based on their exclusive dielectric properties by a novel electrical impedance measurement system Yuqian Zhanga, Esam Salemb, Takahisa Nakamurac and Leyla EsfandiardaOF22.A snorkel-tag based strategy for in vivo isolation of recombinant extracellular vesicles Madhusudhan Reddy Bobbilia, Stefan Vogtb, Severin Muehlederc, Carolina Patriolid, Samir Barbariab, Markus Schossererb, Wolfgang Holnthonee, Heinz Redle, AndrG gensf, Samir El Andaloussig and Johannes Grillarih University of Natural Sources and Life Sciences Vienna (BOKU), Vienna, Austria; bUniversity of Natural Resources and Life Sciences, Vienna (BOKU), Vienna, Austria; cLudwig Boltzmann Institute for Experimental and Clinical Traumatology, vienna, Austria; dUniversity of Organic Sources and Life Sciences, Vienna (BOKU), vienna, Austria; eLudwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria; fKarolinska Institutet, Division of Laboratory Medicine, Stockholm, Sweden; g Department of Laboratory Medicine, Clinical Investigation Center, Karolinska Institutet, IDO Proteins Biological Activity Sweden., Stockholm, Sweden; hChristian Doppler Laboratory on Biotechnology of Skin Aging, University of Organic Resources and Life Sciences, Vienna (BOKU), Vienna, AustriaaUniversity of Cincinnati, Non-hispanic or Latino, USA; bCincinnati Children’s Hospital Healthcare Center, Cincinnati, USA; cCincinnati Children’s Hospiltal Medical Center, Cincinnati, USA; dUniversity of Cincinnati, Cincinnati, USAIntroduction: Exosomes are composed of a lipid bilayer membrane containing nucleic acids, proteins and lipids in the lumen and their compositions reflect their cell of origin. Thus, when the secreting cells are in abnormal microenvironments, the exosomes undergo the compositional modifications. We’ve created a new class of electrical impedance measurement system to non-invasively characterize exosomes based on their special dielectric properties. Even though, the biophysical properties of exosomes which include size, density and shape have been characterized just before, their dielectric properties haven’t been investigated. Strategies: An electrokinetic-based method has been developed to characterize the dielectric properties of exosomes extracted from human hepatocellular carcinoma (HuH-7) cells below unique culture situations. Extracted exosomes have been initially trapped with dielectrophoresis and additional characterized by their dielectric properties as 0.2Vpp was swept from 1 kHz to 50 MHz. Final results: The principle in the impedance measurement was adapted in the Maxwell’s mixing theory applied to analyse the dielectric behaviour of cells. Opacity was defined as the ratio of impedance magnitude at highIntroduction: Extracellular vesicles (EVs) emerged as an importa.