Product Name: Mouse Macrophage
Type: Lysate
Elisa:
WB: 20 μl/lane
Background: Confluent cultures of mouse macrophage cells (J774A.1) were grown to 80% confluency then lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final
CAS NO.: 516-35-8
product targets :　Notch inhibitors
Immunogen:
Buffer And Storage: Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
Specificity:
PubMed ID:http://aac.asm.org/content/51/8/2855.abstract