The nucleosome close to a bin enriched for specific H2A and H3 variants was deemed to be preferentially composed of these two variants.Repeat element sequences ended up attained from Repbase
Anti-FLAG M2 (Sigma, cat# F1804), antiH3K4me3 (Millipore, cat#07-473), anti-H3K27me3 (Millipore, cat#07-449), and anti-H3K36me3 (Abcam, cat# ab9050) antibodies have been utilised for immunoprecipitation. Soon after 3 PBS washes, the antibody-sure beads…