. Differences between samples were considered to be significant at P,0.05. ~~ Airway smooth muscle contraction is mainly controlled by autonomic neurons innervating the airways and the lung. The autonomic…
serum-containing media and coverslips were processed as above. GST-binding Assay GST binding assays were carried out as previously described. Briefly, U2OS cells were lysed in GST lysis buffer or vehicle…
yzed by flow cytometry. The above experiment was repeated five times. Determination of DNA fragmentation in sperm cells The FragELTM DNA Fragmentation Detection assay kit was used to investigate the…
ing in vitro and in vivo The IL-17 pathway has recently been implicated in host defense against a number of both intra- and extra-cellular pathogens. IL17A is known to be…
ion, intracellular thiol levels and enhanced cell survival in oxidative stress. Our data revealed a significant 2-fold Mertansine increase in cellular GSH levels in a-crystallin overexpressing clones when compared to…
ascular protection, anticancer activity and stimulation of proliferation and osteoblastic differentiation in human and mouse MSCs. However, its effects on bone are less studied and are particularly relevant to this…
that activation of GCGR by glucagon also induced the b-catenin signaling pathway. Importantly, we found that Lrp5/6 is required for glucagon-induced b-catenin signaling. These results may help to explain the…
to aid in viral absorption, MgCl2 solution was mixed into sewage and freshwater samples prior to filtration at a final concentration of 25 mM. 100 mL of sewage and 2…
ced b-catenin stabilization and b-catenin-mediated transcriptional activity. Second, inhibiting Lrp5/6 function by a dominant negative construct or a functional inhibitor blocked glucagon-mediated b-catenin signaling. One study showed that PTH1R activated…
ological studies. In vivo metastasis study by intra-cardiac injection Control B16F10 and clone 2 cells were injected intracardiacly into anesthetized C57BL/6 male mice. After 3 weeks, mice were sacrificed; photographed,…