Y an image representing the heel strike event of your correct leg and was ended by the last image RN 1-001 Solubility preceding the next heels strike of the appropriate leg picture (Figure 1A). The animation had a ten Hz frequency. Within the Upside-down condition the same successive images had been merely inverted, keeping the identical kinematics sequences. Within the Uncoordinated condition the first image remained the same because the Regular situation while all the other folks were randomly mixed providing rise to an incoherent Uncoordinated movement. The random sequence was conserved for each trial and each and every subject (Figure 1B). We have calculated the luminosity of each image within the three colors (RGB) by utilizing “imread” MATLab function. This function stores within a matrix the composition in red, green and blue of every single pixel of an image. For every single frame of the animation, we subtracted this matrix to that of the prior frame. We’ve got then computed the imply of the resulting matrix and called this composition “dynamic contrast.” This represents overall adjust involving two successive frames in the composition in luminosity with the same located pixels.Frontiers in Systems Neurosciencewww.frontiersin.orgSeptember 2014 Volume eight Write-up 169 Zarka et al.Neural oscillations in the course of walking observationFIGURE 1 Condition’s stimuli. (A) Time representation on the stimuli in each and every 3 circumstances. Normal condition shows ten successive gait cycles of 1 s every single. Trials were separated by 72 s of gray screen and repeated 22 times. Upside-down condition show the identical pictures sequence, rotated to 180 within the image plane. Uncoordinated conditionshow the exact same initial image as the Standard condition although each of the other people pictures have been mixed. (B) Kinematics representation of your gait within the 3 circumstances. While Typical and Upside-down conditions have the very same coherent kinematic sequence, Uncoordinated condition show an incoherent kinematic sequence.EXPERIMENTAL SETUPThe recording was realized inside a single session. EEG was recorded in 128 channels (ANT method, The Netherlands) at a sampling frequency of 2048 Hz and having a resolution of 22 bits (71.five nV per bit). An active-shield cap making use of 128 AgAgCl sintered ring electrodes and shielded co-axial cables (50 electrode program placements) was comfortably adjusted to subject head. All electrodes have been referred to left earlobe. Impedance was lowered under 10 k for each and every electrode and checked before every single recording. Displays have been presented on a 17 Dell computer screen. Participants looked straight ahead at the computer system screenthrough a form-fitting facemask and a circular barrel (cylinder). The screen was centered around the line of gaze at a distance of 30 cm from the eyes. Viewing by means of the barrel removed any external visual references. Furthermore, subjects had earplugs to isolate from external hearing disturbance. For eight subjects, we presented in three successive sessions the Normal walking situation, then Upside-down walking and then Uncoordinated one (No-Random group). For eight other subjects, conditions were presented randomly in 3 successive sessions (Random group). We created a pause involving every single session so that you can limit the effect of fatigue. Because the aim of this study was to evaluate the impact ofFrontiers in Systems Neurosciencewww.frontiersin.orgSeptember 2014 Volume 8 PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21375761 Write-up 169 Zarka et al.Neural oscillations in the course of walking observationpurposeless perception around the brain rhythms, no unique attentional process was essential. Nevertheless, the state of awareness was continuou.