Tran0.ControlGoAPPAPPDextran0.Fig. 1 The interaction between GoAPP and cell membrane. a Effect of GoAPP on the activity of galactosidase and lactate dehydro genase (LDH) throughout frozen. b Effect of GoAPP around the leakage of intracellular protein and nucleic acid through frozenConcentration from the intracellular protein ( /mL)(a)-galactosidase activity LDH activity(b)1.extracellular nucleic acid extracellular protein1.Chin Med 2018, 13(Suppl 1):Page 55 ofReferences 1. Fearon K, Strasser F, Anker SD, Bosaeus I, Bruera E, Fainsinger RL, et al. Definition and classification of cancer cachexia: an international consen sus. Lancet Oncol. 2011;12(5):489?five. two. Gu C, Qiao J, Zhu M, Du J, Shang W, Yin W, et al. Preliminary evaluation of the interactions of Panax ginseng and Salvia miltiorrhiza bunge with 5fluorouracil on pharmacokinetics in rats and pharmacodynamics in human cells. Am J Chinese Med. 2013;41(2):443?8. 3. Cong M, Zou B, Yu L. Mechanisms of anorexia cancer cachexia syndrome and possible positive aspects of standard medicine and natural herbs. Curr Pharm Biotechnol. 2016;17(13):1147?two.123 Organic fungicides obtained from Linaria species Sophie V. Sokornova, Tatiana V. Matveeva Division of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia Correspondence: Sophie V. Sokornova [email protected] Journal of Chinese Medicine 2018, 13(Suppl 1):123 Background: In natural situations, a few of Linaria species are additional resistant to fungal infection than other Rifamycin S MedChemExpress plants from Antirrhineae tribe [1]. Toadflax plants from sections Linaria and Speciosae contain DNA sequences related to Fenpropathrin manufacturer Agrobacterium rhizogenes T-DNA in their genomes [2]. One of several probable positive aspects of the T-DNA-containing plants is enhanced secondary metabolite production [3]. This function could play part within the resistance of plants to phytopathogens [4]. Plants possess a wide range of protective mechanisms against disease, like a number of types of pre-formed and infection-induced compounds involve phenols/polyphenols (47 ), terpenoids (29 ), alkaloids (11 ) [5]. The most of Linaria species appeared to have high levels of iridoid glycosides, that perform the plant protective function [6?]. Also, L. vulgaris shows higher content of flavonoids with fungicide activity. The aim of this function will be to identify biological activity of key secondary metabolites in Linaria species, representing 3 sections, developing in vitro situations. Materials and methods: Seeds of L. vulgaris, L. genistifolia, L. maroccana were sterilized with H2O2 and placed on Murashige and Scoog [9] medium for germination. Then plants have been propagated by cuttings and cultivated at 24-h photoperiod. Fresh aerial parts of plants were extracted with MeOH for 1 h in an ultra-sonic bath. The crude extract was fractionated with chloroform: methanol in silica gel filled column below vacuum. The fraction was filtered through a Minisart filter sort RC-0.45 , dried and analyzed on GC S. The TLC solvent program utilised was ethyl acetate ethanol ater (38.five:7.five:4). Fungicide activity of extracts was detected by classical agar disk-diffusion strategy (500 mg/ml crude extract, 50 mg/ml fraction) and TLC ioautography strategy [10]. Benefits: Crude aqueous/alcoholic extract from L. vulgaris demonstrated larger fungicide activity than extracts from other species. The highest activity demonstrated antirride enriched fraction (chloroform:methanol four:1). Furthermore, TLC ioautography test have shown the spot with f.