O significant differences amongst meadows and time with regards to the abortedPlants 2021, ten,7 of3.3. Spatio-Temporal Variability inside the Flowering Effort and Reproductive Phenology Flowering work didn’t substantially differ amongst meadows and time (Table three; Figure 5A), displaying an average of 781 157 Scaffold Library custom synthesis spathes m-2 per Z. noltei meadow. Similarly, there have been no important variations among meadows and time regarding the aborted spathes as well as the percentages of spathes in stages I, III and V (Table three; Figure 5B,D,F,G). Plants 2021, 10, x FOR PEER Assessment 15 over On the other hand, the percentage of spathes in stages II and IV have been significantly variableof 15 time (Table 3; Figure 5C,E).Figure 5. Flowering work (A), percentages of spathes in every YTX-465 Data Sheet single reproductive stage (B ) and percentFigure five. Flowering effort (A), percentages of spathes in each and every reproductive stage (B ) and percentage age of aborted spathes (G) time (imply SE, n n = of aborted spathes (G) overover time (mean SE,= four).four).3.4. Germination Ability A total of 158 seeds were obtained from the cultured spathes. Seed production did not differ significantly amongst meadows (M1 2, t = 0.443, p = 0.669; M1 three, t = 0.482, p = 0.643 and M2 three, t = 0.786, p = 0.455), averaging 0.422 0.109 seeds per collected spathe. Amid these seeds, 51.26 (81 seeds) have been fully matured and utilized for the germination testPlants 2021, 10,8 ofTable three. Benefits obtained inside the two-way ANOVA analyses for FE and stages of sexual reproduction. Outcomes are expressed as F-values and associated p-values (in parenthesis). Significant final results are in bold. The significance level in all tests was = 0.01. Variable Flowering effort Stage I Stage II Stage III Stage IV Stage V Aborted spathes Meadow (df = 3) 2.853 (0.047) 1.274 (0.294) 0.408 (0.748) 0.287 (0.834) 1.150 (0.339) 1.266 (0.297) 0.774 (0.514) Time (df = three) 2.495 (0.071) 2.290 (0.090) 10.37 (0.001) 1.470 (0.235) ten.92 (0.001) 1.595 (0.203) 3.375 (0.026) Meadow Time (df = 9) 0.708 (0.699) 1.782 (0.097) 1.010 (0.446) two.282 (0.032) 0.807 (0.612) 1.047 (0.418) 0.534 (0.842)three.4. Germination Potential A total of 158 seeds were obtained in the cultured spathes. Seed production did not differ substantially among meadows (M1 2, t = 0.443, p = 0.669; M1 3, t = 0.482, p = 0.643 and M2 3, t = 0.786, p = 0.455), averaging 0.422 0.109 seeds per collected spathe. Amid these seeds, 51.26 (81 seeds) had been completely matured and utilized for the germination test (Table S2). Similarly, germination capacity didn’t show considerable variations amongst meadows (Table 4), germinating 33.73 of mature seeds. The average germination time with the seeds formed in M1, M2 and M3 was 1.000 0.001, 1.389 0.164 and two.200 0.490, respectively.Table four. Percentage of germinated and no germinated seeds. Comparison among meadows was done by means of Fisher’s precise test of independence. Meadow M1 M2 M3 Fisher’s exact test of independence Germinated 24 43.9 33.three two.69 No Germinated 76 56.1 66.7 p = 0.four. Discussion This study shows the initial data on reproductive phenology of Zostera noltei within the Ria de Aveiro lagoon. The flowering period of Z. noltei in Ria de Aveiro began about June and lasted till near November, peaking between July and August. Flowering effort and reproductive phenology have been similar among meadows and over time, suggesting that all meadows evolved similarly more than the flowering period. The proportion of newly formed spathes (Stage I), apparently fertilised spathes (Stage III) and spathes with mature seeds (Stage V) was co.