Rtelarenlaan 42, 3500 Hasselt, Belgium; 2Maastricht University, dept. Of Physiology, Cardiovascular Analysis Institute Maastricht (CARIM), Universiteitssingel 50, 6200 MD Maastricht, The NetherlandsOPT02.05 = PS05.Proteomic profiling reveal Src as a novel microvesicle-associated biomarker for myocardial infarction Olof Gidl 1, Mikael Evander2, Thomas Laurell1 and David Erlinge1 Lund University, Sweden; 2Department of Biomedical Engineering, Lund University, Sweden; 3Department of Cardiology, Clinical Sciences, Lund University, SwedenIntroduction: Extracellular vesicles (EVs) are a promising source of EphA1 Proteins Purity & Documentation plasma biomarkers for a wide array of disease states, including cardiovascular disease. The principal process for isolating EVs from blood is differential centrifugation, but this technique is time consuming and might compromise the integrity on the vesicles. Acoustic seed trapping is a rapid, non-contact alternative to centrifugation for isolation of EVs from plasma. The aim of this study was to compare the proteomic profiles of EVs from patients with myocardial infarction (MI) and healthy controls isolated with acoustic seed trapping or differential centrifugation employing a proximity extension based assay to recognize novel EV-associated biomarkers for MI.Introduction: EV-mediated intercellular communication amongst monocytes (MC) and endothelial cells (EC) plays an active role in vascular inflammation that in turn can lead to cardiovascular illnesses. The proand anti-inflammatory functional effects of inflammatory EV subpopulations at the site of inflamed vascular cells is poorly understood. As a result, we aim to unravel the pro/anti-inflammatory responses of MC and EC to inflammatory EV. Approaches: TEM, NTA and western blot were utilized to study the size distribution and concentration of UC- purified EV in the culture supernatant of HUVEC, either untreated (uEV)or treated with TNF- to induce an inflammatory strain (tEV). Thereafter, MC and EC in mono and co-culture systems had been exposed for the uEV and tEV. Relevant pro/ anti-inflammatory markers (IL-1, IL4, IL-6, IL8, IL10, IL-13, TNF-, CCR4 Proteins manufacturer ICAM-1, VCAM-1, PECAM-1, E-Selectin, MCP-1, CD40 and HSP70) have been evaluated on RNA level (qPCR) and protein level (ELISA, IF, western blot) in both cell forms. the functionality of uEV and tEV had been assessed applying cell migration and adhesion tests. Results: EV getting an approximate size range between 30-300nm were successfully isolated from EC which could be taken up by MC and EC in culture. We observed that the amount of pro-inflammatory markers (IL-1, IL-6, IL8, ICAM-1, VCAM-1 and MCP-1) in EC and MC treated with tEV at both RNA and protein level have been drastically improved though a substantial decrease in anti-inflammatory marker (IL4, IL10, IL13 and CD40) was detected. We also found that tEV and uEV do induce anti-inflammatory responses in recipient cells as indicated by the enhanced amount of IL4, IL10, IL13 and CD40. Furthermore, tEV promoted both the migration of EC as well as the adhesion of MC to EC. Summary/Conclusion: Taken collectively, our present findings confirmed that each pro and /anti-inflammatory cross talk between EC and MC is established by means of EV-carrying corresponding (RNA and proteins) mediators. Funding: This perform was co-financed by the EU via the Interreg IV Flanders-the Netherlands project VaRiA (IVA-VLANED-3.65) and Interreg V Flanders-the Netherlands project Trans Tech Diagnostics (TTD).Scientific Plan ISEVRoom: Harbour Ballroom Oral with Poster S.