Thelization, and vascularization In vitro in vivo [171]Hyaluronic acid hydrogelGelMA hydrogelOral mucosa regenerationDecellularized AmnioM particlesGelMA AmnioM Particles [172] scaffold has been proven to become effective in neovasculariza tion and mucosa repair In vitro and in vivo Drastically boost burn wound healing [175]Aloe vera gel Skin regenerationSkin regeneration (burn)Nondecellularized mem brane (ROCK Molecular Weight powder) Decellularized hAmnioMNanofibrous FibroinIn vitroBilayer AmnioM/nanofibrous [174] fibroin scaffold represents an efficient natural construct with broad applicability to generate keratinocytes from Menstrual stem cells Decellularized hAmnioM In vitro in vivo The biocompatible scaffold could regenerate both soft and difficult tissue efficiently [192] Web page 12 ofPOC polymerCleft palate repairTable 3 (continued)Goal Periodontal tissue regenera tion Decellularized hAmnioM In vitro Membrane status Study variety Outcome RefEnhancement modalitiesAdditivesElkhenany et al. Stem Cell Analysis TherapyCombination with cellsDental pulp derived cellscell sheet that contained MSC [182] may perhaps be valuable for applica tion in periodontal tissue regeneration Wound healing with a [183] minimal scar within a fullthickness wound in rat back UAM offered a appropriate scaf fold for CSCs to generate tis sue mimic the native cornea AMASCs accelerated the wound healing using a much less inflammatory response within a thirddegree burns rat model High drug entrapment was accomplished by incubation of AmnioM for three h at 4C [193]TGF3 BMSCsSkin regenerationdehydrated AmnioM (hDAM) industrial ultrathin AmnioM In vitro and in vivoIn vitro in vivo(2022) 13:Corneal stromal cells (CSCs)Cornea regenerationASCsSkin regenerationDecellularized hAmnioMIn vitro and in vivo[184]Drug carrier Nanoreservoir Cornea regeneration hAmnioMCefazolinCornea regenerationhAmnioMIn vitro[179]MoxifloxacinIn vitroThick HAM entraps moxi [180] floxacin effectively higher than thin HAM. 3 h incubation was adequate for entrapment Clinical trial (Right after dermoid removal) In vitro and in vivo Fast corneal reepithelization [194] and smooth healing Lysine amino acid could improve the crosslinking efficiency of AmnioM [195]Other additives Cornea regenerationTissue glueCornea regenerationIntact AmnioM Carbodiimide crosslinked AmnioM Decellularized hAmnioMAmino acidsCalcium and PhosphateBone regenerationIn vitro and in vivoThe mineralized AmnioM enhanced ASCs osteogenic differentiation in vitro and bone regeneration in a calva rial bone defect in vivo[181]Page 13 ofElkhenany et al. Stem Cell Research Therapy(2022) 13:Web page 14 ofto facilitate its applications, especially in the corneal defects. Fibrin glue has been PPAR Compound proposed by Szurman, Warga [176] as a bio-adhesive to stabilize the Amnio-M over the corneal surface. Nonetheless, in some instances, like Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) which call for covering the entire cornea, the conjunctiva, at the same time as eyelid, securing a sizable sheet of Amnio-M was challenging. Shanbhag, Chodosh [177] proposed cyanoacrylate glue to fix Amnio-M in to the eyelid skin alongside utilizing a silicon ring to stabilize it over the cornea. One more study around the treatment of recurrent retinal detachment employing Amnio-M has shown that adding platelet-rich plasma (PRP) elevated the success price of sealing the retinal hole [178]. Not too long ago, the drug reservoir properties of your AmnioM happen to be investigated. They were shown to efficiently deliver bioactive molecules like c.