vorable prognosis of TLR2 Purity & Documentation patients XW, FL, and HL conceived and made the study. ZZ and XH download and collated data. YuZ and YiZ performed the statistical evaluation. XW and ZZ performed the information analysis. XW wrote the manuscript. HL revised the manuscript. All authors listed have produced a substantial, direct and intellectual contribution to the operate, and authorized it for publication.FUNDINGThis operate was supported by the National Organic Science Foundation of China (Nos.81472929 and 81172616) to HL.ACKNOWLEDGMENTSWe thank LetPub (letpub) for its linguistic help in the course of the preparation of this manuscript.SUPPLEMENTARY MATERIALThe Supplementary Material for this article might be found on the web at: frontiersin.org/articles/10.3389/fonc.2021. 791943/full#supplementary-materialSupplementary Figure 1 | Correlation analysis featuring carryover threat and model accuracy. (A) Scatterplot amongst C-index and threat effects of clinical traits. (B) Scatterplot between C-index and threat effects of genes.Frontiers in Oncology | frontiersin.orgDecember 2021 | Volume 11 | ArticleWang et al.Dysregulation Activation by Important Gene
animalsArticleSTAT3 manufacturer Transcriptomics Evaluation Reveals the Immune Response Mechanism of Rabbits with Diarrhea Fed an Antibiotic-Free DietLi Chen 1, , Kun Du 1, , Xue Bai two , Jiahao Shao two , Tao Tang 2 , Siqi Xia two , Huimei Fan two , Jie Wang three , Xianbo Jia 3 and Songjia Lai three, College of Animal Science and Technologies, Sichuan Agricultural University, Chengdu 611130, China; chenl2020302120@163 (L.C.); Dukun1672@163 (K.D.) Division of Animal Science, University of Florida, Gainesville, FL 32611, USA; baixue333work@163 (X.B.); shaojh1997@163 (J.S.); m18483220592@163 (T.T.); xiasiqi2020@163 (S.X.); fanhuimei1998@163 (H.F.) Farm Animal Genetic Resources Exploration and Innovation Essential Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China; wjie68@163 (J.W.); [email protected] (X.J.) Correspondence: laisj5794@163 The authors’ contributions would be the very same.Citation: Chen, L.; Du, K.; Bai, X.; Shao, J.; Tang, T.; Xia, S.; Fan, H.; Wang, J.; Jia, X.; Lai, S. Transcriptomics Analysis Reveals the Immune Response Mechanism of Rabbits with Diarrhea Fed an Antibiotic-Free Diet regime. Animals 2021, 11, 2994. doi.org/10.3390/ani11102994 Academic Editors: Mahmoud M. Naguib, Mrigendra Rajput and Mehdi Soltani Received: 1 September 2021 Accepted: 9 October 2021 Published: 19 OctoberSimple Summary: Feeding an antibiotic-free diet program is definitely an inevitable trend in animal husbandry in China. In this study, high-throughput sequencing was made use of to analyze the gene expression variations in several intestinal segments of rabbits. Our analysis identified 168, 593, 2069, 334, 321, and 1423 DEGs inside the comparison groups S_Z (the duodenum of wholesome rabbits) vs. S_B (diarrhea inside the duodenum of rabbits), K_Z (wholesome rabbit jejunum) vs. K_B (rabbits with diarrhea within the jejunum), H_Z (wholesome rabbit ileum) vs. H_B (rabbits with diarrhea within the ileum), M_Z (healthful cecum of rabbits) vs. M_B (rabbit with diarrhea inside the cecum), J_Z (wholesome rabbit colon) vs. J_B (colon of rabbits with diarrhea), and Z_Z (wholesome rabbit rectum) vs. Z_B (rectum of rabbits with diarrhea), respectively. The reproducibility and repeatability on the final results had been validated by RT-qPCR. Enrichment analyses of GO annotations and KEGG pathways revealed the host DEGs which are potentially associated to acute inflammation, strain response,