c procedure, dicarboxylic acid metabolic procedure, and alpha amino acid PAK6 medchemexpress Biosynthetic procedure.two.four. Interaction Networks and Biosynthetic Pathways inside DEGs and DEPs In the outcomes on the DEGs using a log2 fold adjust 3.9, the primary biological function categories have been found to become the leucine catabolic method, L-phenylalanine catabolic procedure, jasmonic acid biosynthetic course of action, response to high light intensity, response to hy6 of drogen peroxide, alpha-amino acid catabolic process, response to heat, response to chitin, 15 response to a virus, and response to a temperature stimulus. The interaction network of proteins encoded by drought-tolerance-related DEGs is shown in Figure 2.Plants 2021, 10,Figure two. Interaction network of proteins encoded by drought-tolerance-related DEGs. The empty nodes represent proteins of an unknown 3D structure, along with the filled nodes signify that some 3D structure is identified or predicted. Edges represent protein rotein associations (bright blue and pink color–known interactions, green and dark blue color–predicted interactions, yellow color–text mining, black color–co-expression). Proteins marked inside the blue square have been drastically associated with drought stress with an adjusted p-value 0.05, and log2 fold transform 3.9. Disconnected nodes or proteins not connected for the major network were hidden inside the network.The evaluation of enriched metabolic pathways (Figure 4) showed some congruences involving transcriptomic and proteomic levels, e.g., tetrapyrrole binding and photosynthetic response normally. On the other side, transcriptomic response was special inside the protein folding/refolding pathway, and in amylase activity. To the contrary, proteomic pathways have been uniquely enriched in responses to oxidative pressure, alkaloid metabolism, and others.Plants 2021, ten, 1878 Plants 2021, ten, x FOR PEER REVIEW7 of 15 8 ofFigure three. Co-expression network of drought-tolerance-related DEPs. The empty nodes represent proteins of an unknown Figure 3. Co-expression network of drought-tolerance-related DEPs. The empty nodes represent proteins of an unknown 3D structure, along with the filled nodes signify that some 3D structure is identified or predicted. Edges represent protein rotein 3D structure, and also the filled nodes signify that some 3D structure is identified or predicted. Edges represent protein rotein associations (bright blue and pink–known interactions, green and dark blue–predicted interactions, yellow–text minassociations (vibrant blue and pink–known interactions, green and dark blue–predicted interactions, yellow–text mining, ing, black–co-expression, multicolor–protein homology). Proteins marked in the red circle were these with an adjusted black–co-expression, marked in the blue homology). Proteins marked SphK1 Formulation within the red circle had been those with an adjusted p-value 0.05. Proteins multicolor–proteinsquare are connected with drought strain. Disconnected nodes or proteins not p-value to the major network within the blue square network. connected0.05. Proteins markedwere hidden within the are associated with drought strain. Disconnected nodes or proteins not connected towards the major network were hidden within the network.Plants 2021, ten, 1878 Plants 2021, ten, x FOR PEER REVIEW8 of 15 9 ofFigure Enriched metabolic pathways below the drought condition in germinating Papaver somniferum plants. A hierarchiFigure 4.4. Enriched metabolic pathways below the drought condition in germinating Papaver somniferum plants. A hierarchical clustering tree summarizin