Iosciences Institute (JHDC, NLG and YSJ) and by a pre-doctoral fellowship
Iosciences Institute (JHDC, NLG and YSJ) and by a pre-doctoral fellowship from ^ CNPq and CAPES by way of the plan `Ciencia sem Fronteiras’ (R E).Further informationCompeting interests XL: A patent application connected to a few of the perform presented here has been filed on behalf with the Regents of the University of California. JHDC: A patent application associated to some of the function presented here has been filed on behalf on the Regents from the University of California. The other authors declare that no competing interests exist. FundingFunder University of California Berkeley Grant reference quantity Author Energy Biosciences Institute Xin Li, Vivian Yaci Yu, Yuping Lin, Kulika Chomvong, Ra sa Estrela, i Annsea Park, Julie M Liang, Elizabeth A Znameroski,Li et al. eLife 2015;four:e05896. DOI: ten.7554eLife.13 ofResearch report Funder Grant reference quantity AuthorComputational and systems biology | EcologyJoanna Feehan, Soo Rin Kim, Yong-Su Jin, N Louise Glass, Jamie HD Cate Conselho Nacional de Desenvolvimento Cient ico i e Tecnologico Ra sa Estrela iThe funders had no role in study style, information collection and interpretation, or the choice to submit the operate for publication.Author contributions XL, VYY, EAZ, JHDC, Conception and design, Acquisition of data, Evaluation and interpretation of information, Drafting or 4-1BB Accession revising the write-up; YL, KC, RE, AP, JML, JF, Acquisition of information, Evaluation and interpretation of data, Drafting or revising the short article; SRK, Analysis and interpretation of data, Drafting or revising the post, Contributed unpublished crucial data or reagents; Y-SJ, Conception and design, Analysis and interpretation of data, Drafting or revising the report, Contributed unpublished important data or reagents; NLG, Conception and style, Analysis and interpretation of data, Drafting or revising the post
Int. J. Mol. Sci. 2013, 14, 24074-24086; doi:ten.3390ijmsOPEN ACCESSInternational Journal ofMolecular SciencesISSN 1422-0067 mdpijournalijms ArticleOptimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic NanoparticlesChi-Yang Yu, Liang-Yu Huang, I-Ching Kuan and Shiow-Ling Lee Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. three, Taipei 10452, Taiwan; E-Mails: (C.-Y.Y.); cherryfish1115gmail (L.-Y.H.); (I.-C.K.) Author to whom correspondence need to be addressed; E-Mail:; Tel.: 886-2-2182-2928 (ext. 6320); 886-2-2585-4735. Received: 9 October 2013; in revised type: 2 December 2013 Accepted: three December 2013 Published: 11 DecemberAbstract: Biodiesel, a non-toxic and biodegradable fuel, has lately turn out to be a significant supply of renewable alternative fuels. Utilization of lipase as a biocatalyst to create biodiesel has advantages over prevalent alkaline IL-15 Formulation catalysts which include mild reaction conditions, uncomplicated product separation, and use of waste cooking oil as raw material. Within this study, Pseudomonas cepacia lipase immobilized onto magnetic nanoparticles (MNP) was used for biodiesel production from waste cooking oil. The optimal dosage of lipase-bound MNP was 40 (ww of oil) and there was small distinction among stepwise addition of methanol at 12 h- and 24 h-intervals. Reaction temperature, substrate molar ratio (methanoloil), and water content (ww of oil) were optimized employing response surface methodology (RSM). The optimal reaction circumstances were 44.two , substrate molar ratio of five.2, and water content material of 12.5 . The predicted and experiment.