Was created up to the mark using the mobile phase to obtain a answer containing 30 /ml of DIC. This solution was utilized for the estimation of DIC. The resolution is further diluted with mobile phase to acquire 2.five /ml MEF and five /ml of PCM, respectively. Both the solutions have been sonicated for 10 min. Options were injected as per the above chromatographic conditions and peak places have been recorded. The quantifications have been carried out by keeping these values to the straight line equation of calibration curve.Results AND DISCUSSIONThe objective with the method development was to resolve chromatographic peaks for active drug components with less asymmetric factor. The mobile phase acetonitrile:20 mM potassium dihydrogen phosphate (70:30 v/v) adjusted to pH four employing orthophosphoric acid was identified to become satisfactory which gave 3 symmetric and wellresolved peaks for DIC, MEF and PCM. The retention instances of DIC, MEF and PCM had been 3.8, 9.3 and 2.5 min, respectively (fig. 1). The resolution among DIC, MEF and PCM was identified to become far more than 2, which indicates PSMA Protein Synonyms excellent separation of all the compounds. The asymmetric things for DIC, MEF and PCM had been 1.36, 1.14, 1.44, respectively. The mobile phase flow price was maintained at 1 ml/min. Overlaid UV spectra of each the drugs showed that DIC, MEF and PCM absorbed appreciably at 220 nm, so detection was carried out at 220 nm.Fig. 1: Chromatogram of normal PCM, DIC and MEF. Chromatogram of common options of paracetamol (PCM, 2.5 min) dicyclomine (DIC, three.8 min) and mefenamic acid (MEF, 9.3 min) obtained in mobile phase. November – December 2014 Indian Journal of Pharmaceutical SciencesijpsonlineLinearity was evaluated by evaluation of working standard options of DIC, MEF and PCM of five unique concentrations as well as the approach was located to be linear inside the selection of ten?00 /ml for DIC, 0.05?0 /ml for MEF and 0.1?0 /ml for PCM, respectively. The regression information obtained are represented in Table 1. Instrument precision was determined by performing injection repeatability test and also the relative common deviation values for DIC, MEF and PCM were found. The intraday and interday precision research were carried out for 3 concentrations of DIC, MEF and PCM plus the results are reported in Table 2. The accuracy from the strategy was determined by calculating recoveries of DIC, MEF and PCM by strategy of standard addition. Recoveries were located to be 97.83?9.26, 98.98?9.53 and 99.79?00.16 for DIC, MEF and PCM, respectively (Table 2). Recovery studies were performed in triplicate. The LOQ for DIC, MEF and PCM have been located to be ten, 0.05 and 0.1 /ml respectively. The LOD for DIC, MEF and PCM were identified to become three, 0.0125 and 0.033 /ml respectively (Table 2). Robustness study was performed by deliberately altering the experimental circumstances like flow price from 1 ml/min to 0.eight ml/min and 1.2 ml/min. The composition of mobile phase was changed varying the proportion of acetonitrile by five . In both the circumstances the recovery of all of the drugs were determined plus the RSD was located to be significantly less than two . Solution stability of DIC, MEF and PCM were evaluated at area temperature for 24 h. All the drugs had been identified to be stable TDGF1 Protein Synonyms having a recovery of more than 98 . Method suitability parameters which include the number of theoretical plates, resolution, and peak assymetry have been determined and reported in Table 2. The proposed system was successfully applied for the determination of DIC, MEF and PCM in their combined dosage form. The recovery was.