05 0.39/0.97 1.01/0.83 1.26/0.65 0.65/1.04 1.10/0.47 0.14/0.99 0.91/1.16 1.26/0.39 1.25/2.12 0.28/1.06 1.26/0.64 1.30/2.26 1.32/0.30 1.32/0.52 1.27/1.25 1.17/0.72 0.91/1.23 0.66/0.87 1.03/1.13 0.82/1.11 0.34/1.1.28/2.37 1.27/0.48 1.30/0.33 0.63/0.92 1.27/0.41 1.17/0.80 1.17/0.76 0.46/1.08 0.24/1.04 0.84/1.06 0.52/1.ten 0.10/1.00 0.21/1.03 1.30/0.27 1.13/0.75 1.30/1.70 1.29/0.30 1.06/1.31 1.27/2.01 1.00/0.85 1.31/3.65 1.22/0.75 0.58/1.11 1.26/1.78 1.24/1.33 1.30/2.46 0.11/0.97 0.93/1.22 0.66/1.09 0.69/1.and significant differentially expressed genes had been examined. As shown in Figure 4A, 4708 differentially expressed genes were detected amongst MP1-vs-MT1, 4947 differentially expressed genes between MP2-vs-MT2, and 4261 differentially expressed genes among MP3-vs-MT3. Figure 4B showed that 2574 differentially expressed genes had been up-regulated and 2134 differentially expressed genes have been down-regulated involving MP1-vs-MT1. There wereup-regulated differentially expressed genes and 2079 down-regulated differentially expressed genes involving MP2-vs-MT2. There have been 2150 up-regulated differentially expressed genes and 2111 downregulated differentially expressed genes involving MP3-vs-MT3. The above results showed that MP2-vs-MT2 had one of the most differentially expressed genes, plus the proportion of up-regulated differentially expressed genes was the largest.Frontiers in Plant Sciencefrontiersin.orgZhang et al.Hederagenin NF-κB ten.Alisertib Epigenetics 3389/fpls.2023.FIGURECluster heatmap of substantial differential metabolite at no cost fatty acids of every group. The six columns in every single heatmap represent the fruits postharvest stages (MP1, MP2, MP3, MT1, MT2, and MT3).three.3 Enrichment evaluation of considerably differentially expressed genesKEGG metabolic pathway evaluation showed that the MP1 vs MT1 comparative group was enriched to 134 pathways; The MP2 vs MT2 comparative group enriched 133 pathways; The MP3 vs MT3 comparative group enriched 133 pathways. As shown in Figure 5, the gene enrichment pathways annotated by the 3 comparison groups are mainly “metabolic pathways”, “biosynthensis of secondary metabolites” and “plant-pathogen interaction”. Based on the above analysis, we further study the distinct functional genomes related to no cost fatty acids.3.four Combined metabolomics and transcriptomic analysis of fatty acid rancidityFatty acid metabolome and transcriptome data had been analyzed jointly in line with KEGG metabolic pathways, and KEGG pathways enriched by both omics have been identified as outlined by various postharvest times (Table three).PMID:23489613 MP1-vs-MT1 was enriched on KEGG into two pathways, Fatty acid biosynthesis and Biosynthesis of unsaturated fatty acids. Palmitoleic acid and Myristic acid were incorporated in fatty acid biosynthesis, and 22 differential genes of this pathway had been annotated. TheABFIGUREOil palm differentially expressed genes statistics of MP and MT. (A) Venn diagram of differentially expressed genes; (B) Histogram of significantly differentially expressed genes.Frontiers in Plant Sciencefrontiersin.orgZhang et al.10.3389/fpls.2023.FIGUREKEGG enrichment classification scatter chart for the pairwise comparisons of MP1-vs-MT1, MP2-vs-MT2, and MP3-vs-MT3.biosynthesis of unsaturated fatty acids pathway contained Arachidic acid, Lignoceric acid, and Behenic acid, and eight differential genes were annotated within this pathway. MP2-vs-MT2 was located to be enriched in five KEGG channels: Fatty acid biosynthesis, Fatty acid metabolism, Fatty acid degradation, Biosynthesis of unsaturated fatty acids,.