Product Name :
Immunoproteasome 20S (human), (purified)
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As indicated on product label or CoA when stored as recommended. Once thawed the material can be stored at +4°C for up to 3 months, or long term at -20°C.
Description:
The eukaryotic proteasome contains 7α-type and 7 β-type subunits. Upon stimulation with IFN-γ, the three active β subunits, β1 (Y), β2 (Z) and β5 (X) are exchanged to their immunocounterparts, namely β1i (LMP-2), β2i (MECL-1) and β5i (LMP-7). This results in a change in proteasomal substrate specificity. Some groups have reported increases in chymotrypsin-like and trypsin-like activities while others have reported no changes or even decreased activities after such treatment. Coomassie stained gel after SDS-PAGE (12.5% acrylamide). Lane (a): molecular weight markers; lane (b) 20S immunoproteosome, spleen-derived (prod.no. BML-PW9645); lane (c): 20S proteosome, erythrocyte-derived (PW8720). All activities were measured by use of 200µM substrate concentration (final concentration) all dissolved in TEAD buffer. Test system: 20µl enzyme solution (640ng); 20µl substrate solution (incubation at 37°C); 200µl stop solution (100mM sodium chloroacetate dissolved in 30mM Na-acetate, 70mM acetic acid, pH 4.{{2332803-84-4} site|{2332803-84-4} Biological Activity|{2332803-84-4} Formula|{2332803-84-4} supplier} 3). Fluorescence was measured in a microplate fluorimeter at 355nm excitation and 460nm emission. Western blot analysis of Immunoproteasome 20S (human), (purified) probed with anti-Syntaxin 3 antibody (ab4113) and anti-Proteasome 20S LMP2 antibody (ab42987). Coomassie stained gel after SDS-PAGE (12.5% acrylamide). Lane (a): molecular weight markers; lane (b) 20S immunoproteosome, spleen-derived (prod.no. BML-PW9645); lane (c): 20S proteosome, erythrocyte-derived (PW8720). All activities were measured by use of 200µM substrate concentration (final concentration) all dissolved in TEAD buffer.{{2364489-81-4} web|{2364489-81-4} Technical Information|{2364489-81-4} In stock|{2364489-81-4} custom synthesis} Test system: 20µl enzyme solution (640ng); 20µl substrate solution (incubation at 37°C); 200µl stop solution (100mM sodium chloroacetate dissolved in 30mM Na-acetate, 70mM acetic acid, pH 4.3). Fluorescence was measured in a microplate fluorimeter at 355nm excitation and 460nm emission. Western blot analysis of Immunoproteasome 20S (human), (purified) probed with anti-Syntaxin 3 antibody (ab4113) and anti-Proteasome 20S LMP2 antibody (ab42987).PMID:30860723
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Additional Information :
| Formulation Liquid. In TEAD buffer (20mM TRIS/HCl, 1mM sodium azide, 1mM DTT, pH 7.4). Contains 50% glycerol. | Purity Detail Highly purified. All starting material has been tested and found to be negative for hepatitis B surface antigen, human immunodeficiency virus type 1 antigens, and antibodies against human immunodeficiency viruses type 1 and 2, and hepatitis C virus. | Source Isolated from human spleen.