Product Name :
MBP Nanoselector Agarose
Applications:
IP,CHIP,MS,Purification
Reactivity :
MBP
Conjugate:
Agarose
Advantages :
· Consistent and reproducible results· No heavy & light antibody chains · Extraordinary binding, even under harsh conditions· High affinity · Short Incubation (5-30 min)
Description:
| Description: MBP Nanoselector Agarose have been specifically designed to bind MBP-fusion proteins. MBP Nanoselector Agarose is based on small high-affinity recombinant alpaca antibody fragments covalently coupled to the surface of Agarose. MBP Nanoselector Agarose is an ideal tool to isolate or purify MBP-fusion proteins fast and efficiently. | Ligand: Anti-MBP single domain antibody fragment (VHH, Nanobody) | Bead size: ~ 40µm | Reactivity: Recognizes MBP or MBP fusion protein. | Binding capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant MBP. | Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze. | Storage | Buffer: 50 % slurry in PBS containing 20 % Ethanol | Background: MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest.{{301326-22-7} medchemexpress|{301326-22-7} Purity & Documentation|{301326-22-7} References|{301326-22-7} supplier} The mechanism by which MBP increases solubility is not well understood.{{2230820-11-6} medchemexpress|{2230820-11-6} Technical Information|{2230820-11-6} Data Sheet|{2230820-11-6} manufacturer} In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.PMID:28723053 The fusion protein binds to amylose columns while all other proteins flow through.MBP Nanoselector Agarose is an affinity resin for immunoprecipitation of MBP fusion proteins. The MBP Nanoselector consists of an MBP Nanobody/ VHH coupled to agarose beads.
Description2 :
MBP Nanoselector Agarose have been specifically designed to bind MBP-fusion proteins. MBP Nanoselector Agarose is based on small high-affinity recombinant alpaca antibody fragments covalently coupled to the surface of Agarose. MBP Nanoselector Agarose is an ideal tool to isolate or purify MBP-fusion proteins fast and efficiently.
Immunogen:
Host :
Isotype:
Purity :
Buffer :
50 % slurry in PBS containing 20 % Ethanol
Storage :
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Function:
Immunoprecipitation (IP)/Co-IP Mass spectrometry (MS) Enzyme activity measurements